discover cell-of-origin and elucidate molecular mechanisms of malignant transformation 

define early pathologic event in disease formation and reveal endothelial and immune cell interactions 

reveal aberrant cellular hierarchy and deregulation of developmental signaling pathways at a single-cell resolution

identify rare cell population and aberrant changes in diseased samples

detect transcriptomic changes in specific cell types with spatial information

validate key targets and pathways using human samples, mouse models, and in vitro culture system